HIGH-QUALITY PEPTIDES - DELIVERED ANYWHERE IN CANADA

Add your Title Here

Proper Peptide Reconstitution

Peptides are sensitive biomolecules that require rigorous handling and storage to maintain their integrity and efficacy. Our peptides are delivered as lyophilized (freeze-dried) powder, which gives them great stability against short-term temperature variations during transport. Even at room temperature, these powders remain stable for several weeks. However, to maximize their shelf life and ensure consistent results, proper storage and reconstitution methods must be followed.

Best Practices for Storage and Handling

Before Reconstitution
Store lyophilized peptides in a dry and cool place. For long-term storage, use a freezer set between -20°C and -80°C, to avoid any degradation or contamination.

After Reconstitution
Once reconstituted, peptides become more fragile.

  • For short-term use (a few days), store the solution at 4°C.
  • For prolonged use, divide it into aliquots and freeze them at -20°C.
    Absolutely avoid repeated freeze-thaw cycles, as this reduces their bioactivity.

How to Properly Reconstitute a Peptide

Choosing the Right Solvent

The choice of solvent depends on the peptide’s solubility:

  • Sterile Bacteriostatic Water: often used, but may be insufficient for hydrophobic peptides. Order here
  • 0.1% acetic acid or diluted HCl: useful for certain hard-to-dissolve peptides.
  • pH-adjusted solvents: necessary in certain specific cases.

Add the Solvent Gently

  • Pour the solvent gently along the side of the vial.
  • Do not violently shake the vial: this can degrade the molecule.
  • Ensure the solution is clear and free of visible particles.

Common Errors to Avoid

  • Using tap water or non-sterile water (risk of contamination).
  • Shaking the vial aggressively.
  • Leaving the reconstituted solution at room temperature for too long.
  • Not dividing into aliquots, leading to repeated freeze-thaw cycles.

In Summary

Proper reconstitution and storage ensure the stability, potency, and reliability of your peptides for all your experimental applications. Adhering to these best practices is essential for consistent and reproducible research results.

0
    0
    Your Cart
    Your cart is empty